survival, proliferation, and migration of human meningioma stem-like cells in a nanopeptide scaffold

objective(s): in order to grow cells in a three-dimensional (3d) microenvironment, self-assembling peptides, such as puramatrix, have emerged with potential to mimic the extracellular matrix. the aim of the present study was to investigate the influence of the self-assembling peptide on the morphology, survival, proliferation rate, migration potential, and differentiation of human meningioma stem-like cells (hmgscs). materials and methods: the efficacy of a novel method for placing hmgscs in puramatrix (the injection approach) was compared to the encapsulation and surface plating methods. in addition, we designed a new method for measurement of migration distance in 3d cultivation of hmgscs in puramatrix. results: our results revealed that hmgscs have the ability to form spheres in stem cell culture condition. these meningioma cells expressed gfap, cd133, vimentin, and nestin. using the injection method, a higher proliferation rate of the hmgscs was observed after seven days of culture. furthermore, the novel migration assay was able to measure the migration of a single cell alone in 3d environment. conclusion: the results indicate the injection method as an efficient technique for culturing hmgscs in puramatrix. furthermore, the novel migration assay enables us to evaluate the migration of hmgscs.